Composite

Part:BBa_K3190601:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-16)


pGAL1-BI-I: BI-I CDS under inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence of BAX Inhibitor-I anti-toxin under the control of inducible promoter for the design of a kill switch system for a yeast wherein a BAX toxin is expressed under a constitutive promoter. This system ensures that the yeast survives only unless it is present in the medium with inducer.


Source

Promoter from S. cerevisiae while BI-I cds from Homo sapiens

References